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"Major"
- Akwesasne Thunder
- Brampton Excelsiors
- Brooklin Redmen
- Ohsweken Wolves (Operations suspended for 2000)
- Peterborough Lakers
- Six Nations Chiefs
- St.Catharines Athletics
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Jr "A"
- Brampton Excelsiors
- Burlington Chiefs
- Kitchener-Waterloo Braves ( K-W )
- Mississauga Tomahawks
- Orangeville Northmen
- Orillia Kings
- Peterborough Javelins
- Six Nations Arrows
- St.Catharines Athletics
- Toronto Beaches
- Whitby Warriors
East ![]()
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Jr "B"West
- Akwesasne Lightning
- Barrie Tornado
- Gloucester Griffins
- Green Gaels ( Clarington )
- Kahnawake Eagles
- Mimico Mountaineers
- Nepean Knights
- Onondaga Jr Warriors
- Scarborough Saints
- Elora Mohawks
- Guelph Royal City Regals
- Halton Hills Bulldogs
- Milton Mavericks (Operations suspended for 2000)
- Owen Sound Flying Dutchmen
- Sarnia Pacers
- Six Nations Red Rebels
- Spartan Warriors
- Wallaceburg Red Devils